
Troy University teacher and plant manager Curren Farmer formed the idea for the Pioneer Museum of Alabama in the late 1960s as a way to save old farm equipment so future generations could learn about their ancestors' lives. With the help of his wife, Margaret Farmer, a historian and author, and several friends, Farmer petitioned the nearby community of Troy to donate old farm equipment as well as land on which to exhibit it. In 1969, the Farmers established the Pike County Pioneer Museum and continued to accept donations of various agricultural artifacts, including tractors, plows, and other farming implements. During this time, the Gibson family donated land for the museum, and in 1971, the Farmers opened the Pioneer Museum to the public.

The most notable structures in the museum's collections are its two cabins. The Dogtrot House, originally built by Thomas Madison in Pike County in 1830, is an example of a typical nineteenth-century pioneer home. The house has two main rooms separated by an open hall, or "dogtrot," that aided in airflow and cooling in Alabama's hot environment. The one-room Tenant House has wooden shingles covering the outside, and the interior walls are insulated with cardboard and newspapers, some of which date back to the Spanish-American War. It is an example of the type of dwelling used by sharecroppers of the late nineteenth and early twentieth centuries. In addition to the cabins, the museum's collection of historical structures includes a church and a covered bridge from the nineteenth century.

The museum is heavily involved in Pike County events and offers many programs in conjunction with county festivities throughout the year. The museum hosts three annual events as part of its mission to educate the public about Alabama's pioneer history: Pioneer Days, Spring Plantin', and Old Time Christmas. Pioneer Days is a weekend event in the fall that includes demonstrations of nineteenth century chores such as woodworking, blacksmithing, rope-making, spinning, and quilting. In addition, visitors can attend Wild West shows, Civil War reenactments, and retellings of the "Legend of Davy Crockett." Spring Plantin' is a weekend event that is held in conjunction with the Pike County Master Gardeners Association. Museum staff plant a spring garden and the Master Gardeners host a spring plant sale. Staff and volunteers also host nineteenth-century games such as "whinididdles," "whirligigs," "Jacob's ladder," and "hoop and sticks" for children. Old Time Christmas is a winter holiday-themed event in December in which staff and volunteers demonstrate how Christmas was celebrated in the eighteenth and nineteenth centuries. Visitors can try hot chocolate made on a wood stove, roast marshmallows, sing Christmas carols in the church, and visit a holiday-themed gift shop.
The museum has a staff of approximately seven individuals overseen by an executive director and a 12-member board of directors. The facilities are available to rent for a variety of occasions.